Department of Biochemistry - University of Arkansas-Fayetteville
Thursday, April 20, 2017
4:10 p.m. - 1414 Molecular Biology Building
Host: Tom Bobik
"Upgrading Protein Synthesis with Non-canonical Amino Acids"
In the past few years, more than 200 noncanonical amino acids (ncAAs) have been incorporated into proteins by the genetic code expansion strategy, which uses an orthogonal pair of aminoacyl-tRNA synthetase (AARS) and tRNA (no crosstalk with endogenous AARSs and tRNAs in hosts) to incorporate an ncAA in response to a stop codon at the designed position in the protein of interest. This strategy provides powerful tools for protein studies, enabling the incorporation of photo-crosslinkers for mapping weak or transient protein interactions, post-translational modifications for studying their functions and identifying modifying enzymes, photo-caged UAAs for controlling biological processes by light, as well as biophysical probes and labels for providing insights into how proteins respond to stimuli. Here, the development of the genetic code expansion technique will be reviewed, the challenges in this field and how to overcome will be discussed, and practical applications in biological studies will be demonstrated.
BBMB GRADUATE STUDENTS ARE REQUIRED TO ATTEND!
PLEASE JOIN US AT 3:45 P.M. IN THE MBB ATRIUM FOR REFRESHMENTS