Yifan Cheng Seminar

Department of Pharmaceutical Sciences - University of California-Irvine

Thursday, March 23, 2016
4:10 p.m. - 1414 Molecular Biology Building
Host: Dipali Sashital

"Single Particle Cryo-EM of Membrane Proteins – A Breakthrough in Structural Biology"

My laboratory developed a novel method to correct for electron beam-induced image motion in single particle cryoEM datasets by using a direct electron counting camera, which enabled us to determine a 3.3Å three-dimensional (3D) reconstruction of the archaeal 20S proteasome. This is the first 3D reconstruction at near atomic resolution for a particle of this size. Utilizing these novel cryo-EM technologies, we determined the structure of the Transient Receptor Potential vanilloid receptor 1 (TRPV1) ion channel at 3.4Å resolution by single particle cryoEM. This is the first atomic structure of an integral membrane protein determined by single particle cryoEM without the use of crystals, and also the first atomic structure of the TRP ion channel superfamily. We determined the atomic structure of TRPA1, which is a member of another TRP channel subfamily. Recently, we succeeded in determining atomic resolution structure of TRVP1 in lipid nanodisc, facilitating structural studies of lipid-protein interaction by single particle cryo-EM. In addition, my laboratory developed a novel method which uses Fab (Fragment antigen-binding) fragments to assist single particle cryoEM studies of small integral membrane proteins.

BBMB GRADUATE STUDENTS ARE REQUIRED TO ATTEND!
PLEASE JOIN US AT 3:45 P.M. IN THE MBB ATRIUM FOR REFRESHMENTS