Department of Pharmaceutical Sciences - University of California-Irvine
Thursday, February 23, 2017
4:10 p.m. - 1414 Molecular Biology Building
Host: Walter Moss
"Progress and Challenges for Chemical Probing of RNA Structure Inside Living Cells"
The advent of deep sequencing technology has unexpectedly advanced our structural understanding of molecules composed of nucleic acids. A significant amount of progress has been made recently extrapolating the chemical methods to probe RNA structure into sequencing methods. Herein we review some of the canonical methods to analyze RNA structure, and then we outline how these have been used to probe the structure of many RNAs in parallel. The key is the transformation of structural biology problems into sequencing problems, whereby sequencing power can be interpreted to understand nucleic acid proximity, nucleic acid conformation, or nucleic acid-protein interactions. Utilizing such technologies in this way has the promise to provide novel structural insights into the mechanisms that control normal cellular physiology and provide insight into how structure could be perturbed in disease.
Research in the Spitale lab is funded by the University of California, the American Cancer Society, NIH Director’s New Innovator Award (1DP2GM119164) and 1R01MH109588.
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